Journal: OncoImmunology

Article Title: An epitope-specific novel anti-EMMPRIN polyclonal antibody inhibits tumor progression

doi: 10.1080/2162402x.2015.1078056

Figure Lengend Snippet: Figure 1: EMMPRIN structure and peptide design, and specificity of 161-Ab: (A) Partial

Article Snippet: One strip was probed with the 1:1,000 diluted commercial rat anti-mouse EMMPRIN (MAB772, R&D systems) and then with the 1:5,000 diluted HRP-conjugated goat anti-rat IgG (112-035- D ow nl oa de d by [ U ni ve rs ity o f R eg in a] a t 0 6: 58 2 4 Fe br ua ry 2 01 6 062, Jackson) and served as positive control (P.C).

Techniques:

Journal: PLoS ONE

Article Title: Extracellular Vesicles Secreted from Cancer Cell Lines Stimulate Secretion of MMP-9, IL-6, TGF-β1 and EMMPRIN

doi: 10.1371/journal.pone.0071225

Figure Lengend Snippet: A) Size exclusion chromatography elution profile of the purification of conditioned media to isolate EVs. The red box corresponds to the elution peak for the purified EVs. B) EMMPRIN is secreted via EVs in all cell lines tested as shown by the Western blot probed for EMMPRIN in the vesicle fractions purified using size exclusion chromatography. C) NanoParticle Tracking analysis detects EVs in the purified sample and validates our size exclusion method to purify EVs from conditioned media. D) Electron microscopy was used to visualize the secreted EVs. Data shown is for EV MDA .

Article Snippet: 10 μg of Human EMMPRIN Biotinylated polyclonal antibody (R & D Systems, Minneapolis, MN, USA) was used to immunoprecipitate (IP) EMMPRIN from each sample.

Techniques: Size-exclusion Chromatography, Purification, Western Blot, Electron Microscopy

Journal: PLoS ONE

Article Title: Extracellular Vesicles Secreted from Cancer Cell Lines Stimulate Secretion of MMP-9, IL-6, TGF-β1 and EMMPRIN

doi: 10.1371/journal.pone.0071225

Figure Lengend Snippet: Fluorescence microscopy images of THP-1 cells treated with Texas red stained EVs. Left panel - THP-1 cell stained with EMMPRIN FITC and DAPI. The red signal is the auto-fluorescence signal of the cell. Middle panel - THP-1 cell treated with the Texas Red stain alone. The signal observed is the same as in the left image and corresponds to the Texas red background and cell auto-fluorescence signal. Right panel - THP-1 cells treated with Texas Red stained EVs and visualized after a 5-minute incubation period. The cell membrane is stained with EMMPRIN-FITC antibody, the EVs are shown in red and the DAPI stained nucleus is shown in blue. The reference bar is 10 µm.

Article Snippet: 10 μg of Human EMMPRIN Biotinylated polyclonal antibody (R & D Systems, Minneapolis, MN, USA) was used to immunoprecipitate (IP) EMMPRIN from each sample.

Techniques: Fluorescence, Microscopy, Staining, Incubation, Membrane

Journal: PLoS ONE

Article Title: Extracellular Vesicles Secreted from Cancer Cell Lines Stimulate Secretion of MMP-9, IL-6, TGF-β1 and EMMPRIN

doi: 10.1371/journal.pone.0071225

Figure Lengend Snippet: Western blot analysis of THP-1 cells shows the change in EMMPRIN secretion with and without stimulation with EV MCF-7 and EV MDA .

Article Snippet: 10 μg of Human EMMPRIN Biotinylated polyclonal antibody (R & D Systems, Minneapolis, MN, USA) was used to immunoprecipitate (IP) EMMPRIN from each sample.

Techniques: Western Blot